Distribution of Arbovirus Antigens in Density Gradients
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Sucrose gradient analysis of Venezuelan equine encephalitis virus, a group A arbovirus, indicated that hemagglutinating HA and complement-fixing CF activities were coincident with virus infectivity. In contrast, West Nile, Ilheus, and Rio Bravo viruses of group B were fractionated into infectious and noninfectious components. The infectious component sedimented as a single entity, possessed HA and CF activity, and had a buoyant density of l.19 gcc. West Nile and Ilheus virus suspensions contained a slower sedimenting noninfectious HA and CF component with a buoyant density of 1.16 gcc. Rio Bravo virus suspensions did not contain a similar component. A very slow-sedimenting noninfectious CF component 6 S, 1.10-1.12 gcc was detected for each of the group B viruses.