Studies on Mammalian and Human Pyruvate and alpha-Ketoglutarate Dehydrogenation Complexes.
Rept. no. 10 (Annual), 15 Mar 70-14 Mar 71.
NAGASAKI UNIV (JAPAN) ATOMIC DISEASE INST
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Enzyme systems that catalyze a coenzyme A- and nicotinamide adenine dinucleotide-linked oxidative decarboxylation of pyruvate and 2-oxoglutarate have been isolated from escherichia coli as highly organized multienzyme complexes with high molecular weights by Koike et al. in 1960, and the pyruvate dehydrogenase complex was further separated into three constituent enzymes by Koike et al. in 1963. The pig heart pyruvate and 2-oxoglutarate dehydrogenase complex were isolated in highly purified states as multienzyme units with molecular weights to several million. The objectives of the research are to resolve the pig heart pyruvate and 2-oxoglutarate dehydrogenase complexes into their essential constituent enzymes, to characterize the latter, and to determine the composition and macromolecular structure of these two multienzymes. Author