Hemoglobin Function in Stored Blood: VIII. Further Effects of Phosphate on Red Cell ATP and 2,3-DPG.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
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Inorganic phosphate which is known to stimulate red cell glycolysis is present in one of the preservatives for storing whole blood, citrate-phosphate-dextrose CPD, but not the other, acid-citrate-dextrose ACD. Both of these preservatives for liquid storage were developed before 2,3-diphosphoglycerate 2,3-DPG was found to be necessary for normal hemoglobin function. In a recent study we have shown that very high concentrations of phosphate 10, 15, and 20 mM were deleterious for maintaining 2,3-DPG. In the present study a lower range of phosphate concentrations 2, 4, 6, and 9 mM was studied for maintenance of 2,3-DPG and ATP during storage under blood banking conditions. The lowest concentration, 2 mM, which corresponds to CPD was found to be the best concentration for maintaining 2,3-DPG and thus hemoglobin function. Four mM phosphate were not quite as good but better than no phosphate. Six and 8 mM phosphate were considerably worse. Author