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Metabolic and Functional Characteristics of Red Blood Cells with Extreme Elevation of 2,3-Diphosphoglyceric Acid.
Final rept. 1 Nov 68-30 Apr 71,
FLORIDA UNIV GAINESVILLE
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Anticipating a possible role for transfusion of red cells with elevated concentrations of 2,3-diphosphoglycerate 2,3-DPG in certain clinical situations, the metabolic and functional properties of red cells with very high levels of 2,3-DPG have been investigated. The 2,3-DPG content of red cells was elevated by incubation with inosine, pyruvate, and phosphate IPP at both 37C and 4C. 2,3-DPG was found to be surprisingly stable, persisting at 5 x normal levels for 24 hours after removal of IPP. Glycolysis was markedly reduced, primarily by inhibition of hexokinase. The rate of deoxygenation of the red cells in the presence of dithionite is markedly increased. This effect has been shown to be mediated by the low intracellular pH induced by elevation of 2,3-DPG. In addition, a direct effect of 2,3-DPG ON DEOXYGENATION RATE AND THE Bohr effect of red cells has been demonstrated. Author
APPROVED FOR PUBLIC RELEASE