Studies on the Biogenesis and Chemistry of the Adhesive Substance Secreted by the Cyprid and Metamorphosed Stages of the Barnacle - Balanus.
Progress rept. Mar-Jun 71,
NEW YORK AQUARIUM BROOKLYN OSBORN LABS OF MARINE SCIENCES
Pagination or Media Count:
Several new approaches have been used to study the biogenesis, and the chemical and physical properties of the barnacle cement. An extensive study on the histochemistry of the adhesive substance in the fluid and solid states of the pre- and post-metamorphosed cyprid of Balanus eburneus has recently been completed and will be published in the near future. This study shows that tryptophan is one of several alpha-amino acids present in the intracellular cement. Of special interest is the discovery of Arylsulphatases ASase A, B, C in the mantle and other tissues of the barnacle this is the first time that this class of enzyme has been found in crustacea, and in particular, the Cirripedia. Most of the work in this study was concerned with details on the kinetics and physiological properties of the enzymes. One observation of particular importance is the fact that ASases are inhibited by sulphate, sulphite and phosphate ions, but not by cyanide. Studies are presently underway to establish the interrelationships of ASases, other enzymes and molting hormones in the biogenesis, polymerization and hardening of the cement substances.
- Adhesives, Seals and Binders