Prognostic Significance of Concentrations of Four Classes of Protein-Bound Carbohydrates in the Serum of Dogs
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Three groups of eight dogs each were given doses of 225, 230, and 400 rads of mixed gamma-neutron radiation, respectively. The doses were delivered at a rate of approximately 20 radsminute from the AFRRI-TRIGA reactor. In animals with poor prognosis, the serum protein concentrations milligrams carbohydrate per 100 mg biuret protein of neutral hexoses, hexosamines, and sialic acid started to rise at varying times postirradiation, continued upward, and remained high until the death of the animal. Moderate to marked fluctuations in the protein concentrations were seen in the time course of all these carbohydrates, especially in the two lower dose groups. The protein concentration of the neutral hexoses gave the clearest warning of unfavorable prognosis in the animals which died and exhibited the greatest stability in those which survived. No significant changes in the concentration of protein- bound fucose were seen in any of the 24 dogs. Serum protein-bound neutral hexose concentrations offer promise for development of a relatively simple, objective prognostic test to supplement clinical observation in cases of radiation injury. Thus, numerical ranges of protein concentrations of neutral hexoses are proposed to indicate good, guarded, and poor prognoses for this species. These tentative ranges were tested in nine additional dogs and were found to be effective for prediction.