Ultrafiltration as a Method for Concentrating Rift Valley Fever Grown in Tissue Culture
FORT DETRICK FREDERICK MD
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Filtration by means of a Diaflo ultrafilter was used to concentrate three 1,000-ml lots and one 3,000-ml lot of tissue culture-grown Rift Valley fever virus. Quantitation of both infectivity and total protein was achieved. Water treatment with continued ultrafiltratin of the virus concentrate provided a final virus product approximately 99.25 free of low-molecular-weight materials originally present in the growth medium.