Acetylcholinesterase Activity in Synaptic Vesicles of the Medulla of Rats Exposed to High Doses of Gamma-Neutron Radiation
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Sprague-Dawley rats were subjected to a whole-body dose of 15 krads of mixed gamma-neutron radiation. At 2 and 24 hours following irradiation 14 animals from the irradiated group and 14 unirradiated controls were sacrificed by replacing the blood in the brain circulation by perfusion with a sucrose- eserine solution. The medulla section of the brain was removed, homogenized and prepared for ultracentrifugation and sucrose density gradient separation. The separated synaptic vesicles were assayed for protein and acetylcholinesterase ACHE. A decrease in protein was found 2 hours after irradiation with a greater decrease 22 hours later. A decrease in ACHE activity was also seen 2 hours after irradiation but there was no further decrease at 24 hours. These results were correlated with vascular changes found during the central nervous system CNS syndrome.
- Anatomy and Physiology