Fluorescence Spectra, Polarization, and Life Times of Biological Compounds in Living Cells and in Model Systems.
Rept. no. 2 (Annual) 1 Jan-31 Dec 70,
CONNECTICUT UNIV STORRS DEPT OF ANATOMY
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The purpose of this experimentation is to extract molecular information about the interaction between a variety of chemical substances of biologic interest with intact individual living cells studied at the microscope level. The goal of the work is to seek the nature of in vivo complexing by compounds whose mechanism of binding is not fully understood to clarify the effects of agents known to have biologic action, therapeutic or toxic. By special instrumentation, three parameters of fluorescence spectrum, decay time, and polarization are obtained from the complexes formed in the cells. The analysis of the three parameters can produce data detecting electronic energy transfer mechanisms, states of molecular orientation, molecular volume of complexes, certain degrees of local freedom and the presence or absence of macromolecule formation. This years report contains discussion of the progress and problems with the decay time and polarization equipment and presents cell studies done with the equipment on a fluorescent probe for membrane structure ANS, a polycyclic hydrocarbon, and on antimalarial drug fluorescence. Author