An Automated Analysis of Creatine Phosphokinase Not Requiring Dialysis
NAVAL SUBMARINE MEDICAL RESEARCH LAB GROTON CT
Pagination or Media Count:
A method, designed for simplicity, has been described for the automated analysis of creatine phosphokinase. The procedure is not only applicable for measurement of serum enzyme activity, for which the analysis of this enzyme serves its most important clinical function, but also for the activity of a wide variety of other tissues. Since no protein precipitation or dialysis step is required by the method, minimal equipment is needed and significant economies are effected in sample size and reagent utilization. A combined analytical procedure for creatine phosphokinase and lactic dehydrogenase activities is described and the clinical importance of these techniques when employed together is briefly discussed. This method was devised specifically to apply to the enzymatic evaluations of the effects of submarine and diving environments.