Studies of the Use of Hemopoietic Marrow Transplants in Inducing Osteogenesis in the Surgical Repair of Defects of the Facial Bones.
Final progress rept.,
CALIFORNIA UNIV LOS ANGELES SCHOOL OF DENTISTRY
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A laboratory method of investigation has been developed for the study of the interface between transplants of hemopoietic marrow and other types of graft materials and the soft tissues and osseous surfaces of the recipient host. This test system involves the use of tissue chamber implants lined with a cellulose acetate filter membrane which permits tissue fluid profusion but which prevents cellular interchange between the hose and the graft. By the fluorescence microscopy of teteracycline induced fluorescence produced by the parenteral injection of th host recipient it has been possible to quantify new osseous matrix formation within the implanted chambers under various environmental circumstances. The osteogenic effect of the addition to autogenous marrow implants of calcified matrix in the form of freeze-dried homografts, surface-decalcified homografts, and fresh and frozen autogenous cancellous bone has been studied. It was found that calcaified matrix from immunologically acceptable sources has the ability to influence the osteogenic function of the marrow transplants. These studies have shown that the most optimal types of calcified matrix capable of enhancing the osteogenic phenomenon in marrow transplants are cancellous autogenous bone and surface decalcified homogenous bone. Additionally, this work has indicated that autogenous cancellous bone and marrow may be stored at low temperatures after programmed freezing with apparent preservation of the osteogenic potential. Author
- Medicine and Medical Research