Accession Number:

AD0719995

Title:

Benzoylated DEAE-Sephadex: Its Preparation and Application,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Report Date:

1970-08-17

Pagination or Media Count:

4.0

Abstract:

In the goal of synthesizing a DNA molecule for biological studies, large amounts of protected di- and trinucleotides were required. The large scale syntheses 2-10 mmoles of these starting blocks required chromatography through large columns of benzoylated DEAE-cellulose. This is a time-consuming and laborious step. In order to overcome this difficulty, the authors investigated the benzoylation of DEAE-Sephadex with a view to produce an adsorbent of high capacity. In this communication we wish to report the successful preparation of benzoylated DEAE-Sephadex and also its application for the large scale synthesis of protected oligonucleotides. Author

Subject Categories:

  • Biochemistry
  • Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE