Hemoglobin Function in Stored Blood. VI. The Effect of Phosphate on Red Cell ATP and 2,3-DPG.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
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The objective of the study was to determine if a higher concentration of inorganic phosphate than 2mM CPD citrate-phosphate-dextrose would benefit red cell concentrations of ATP and 2,3-diphosphoglycerate during blood storage. Blood preservatives with phosphate concentrations of 2 mM CPD, 5 mM, 10mM, 15mM, and 20 mM were used to study red cell preservation under blood bank storage conditions at 4 C. Procedural and analytic methods previously employed in these laboratories were employed. Concentrations of phosphate higher than 2 mM CPD were found to be of no benefit for maintenance of ATP and 2,3-DPG during 5 weeks of storage. Author