The Action and Interaction of Purified Lymphoid Cells in the Rejection of Skin Grafts.
Final rept. Mar 68-Jun 70,
HEKTOEN INST FOR MEDICAL RESEARCH CHICAGO ILL
Pagination or Media Count:
Using static cultures, spleen cells taken from immunized animals in several different oxygen concentrations have been maintained. Outside of atmospheric conditions, the amount of oxygen dissolved in the tissue culture media does not reach the level of that in the gas phase. High oxygen tensions have been shown to have different effects on cells explanted during a primary or secondary immune response. Cells involved in the primary response are not susceptible to high oxygen tensions, however those taken during a secondary response to the same antigen, sheep erythrocytes are susceptible. The number of direct and indirect hemolytic plaques drop off more rapidly with time in culture when the cells are maintained in high oxygen concentrations. The susceptibility to high oxygen tensions is also evident during the secondary response to Keyhole Limpet hemocyanin, again by both direct and indirect plaque assays, as well as by passive hemagglutination. This suggest that different populations of cells or cells with different physiologic requirements are operative during the primary and secondary response. Cell growth per se is not indicative of function, and some of the cells which survive and proliferate at high oxygen tensions may be distinct from those involved in the antibody response. Author
- Medicine and Medical Research