Disseminated Intravascular Coagulation and Renal Failure: Production in the Monkey with Autologous Red Cell Stroma
ARMY MEDICAL RESEARCH LAB FORT KNOX KY FORT KNOX
Pagination or Media Count:
Transfusion of incompatible blood may result in disseminated intravascular coagulation DIC and renal failure. Recent studies in our laboratories have shown that red cell stroma free hemoglobin infusions are free of these effects. This report is concerned with the role of red cell stroma in the production of these abnormalities. Sonicated autologous red cells derived from 25 of calculated blood volume were infused into cynomolgus monkeys. DIC, characterized by depression of platelet counts, fibrinogen, and Factors V and VIII occurred in every case. Autopsy of two animals that died after infusion disclosed hemorrhagic pneumonia and alterations in renal architecture. Sonicated red cell stroma prepared from red cell ghosts obtained from the same volume of blood caused a similar but less severe reaction. Glomerular filtration, as measured by clearance of inulin, and endogenous creatinine fell transiently in both groups. Control animals infused with 0.85 saline exhibited no significant changes in these tests. This study supports the view that DIC and subsequent renal failure following intravascular hemolysis of red cells are due to the stromal fraction of the cell.
- Medicine and Medical Research