DETERMINATION OF ALPHA-TOCOPHEROL IN FREEZE-DRIED FOODS BY A MODIFIED COLORIMETRIC PROCEDURE.
Final rept. 1 Oct 69-30 Apr 70,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
Pagination or Media Count:
A reliably precise and reproducible determination of alpha-tocopherol was achieved by an adaptation of the classic colorimetric determination of alpha-tocopherol by Emmerie and Engel. The method was preceded by the separation of the various tocopherols either by secondary magnesium phosphate column chromatography as described by Bro-Rasmussen and Hjarde, and Freed or by Florisil column chromatography according to the method of Dicks-Bushnell. The basic Emmerie-Engel method, despite its disadvantages, is considered the most suitable method for use with oils, foods, and feeding stuffs. Samples of precooked, freeze-dried ground beef, chicken cubes, whole eggs, corn, beans, and peas were analyzed and alpha-tocopherol values consistent with those reported in the literature were obtained. Recovery values achieved with the addition of pure alpha-tocopherol ranged from a minimum recovery of 90.2 to a maximum recovery of 99.1. Author
- Food, Food Service and Nutrition
- Physical Chemistry