Accession Number:

AD0712961

Title:

A BIPHASIC EXTRACTION FOR 2,8-DIOXYADENINE.

Descriptive Note:

Final rept.,

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

• White,Sandra H.
• Loken,Michael R.
• Shields,Charles E.

Report Date:

1970-04-10

Pagination or Media Count:

11.0

Abstract:

A rapid quantitative method for 2,8-dioxyadenine in biological fluids has been developed using a biphasic HCl extraction from Dowex-50W, X-8, 200-400 mesh, hydrogen form. The precision and accuracy of this assay were evaluated. The least square fit of the standardization gives a slope of 0.999 plus or minus 0.0647 resulting in essentially 100 recoveries. When compared with the 2-day column chromatography technique previously available, this procedure permits numerous determinations within the hour. The result is a rapid screening assay for 2,8-dioxyadenine whenever large doses of adenine are administered. A variation of this procedure may be used as a semiquantitative test for adenine determination. Author

Descriptors:

• (*ADENINE
• *ION EXCHANGE)
• PURINES
• KETONES
• URINE
• BLOOD
• STORAGE

Subject Categories:

• Biochemistry
• Physical Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE