Accession Number:

AD0712613

Title:

ROLE OF INTERFERON IN THE PROPAGATION OF MM VIRUS IN L CELLS

Descriptive Note:

Corporate Author:

SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX

Personal Author(s):

Report Date:

1969-08-18

Pagination or Media Count:

8.0

Abstract:

MM virus propagated in mouse brain replicates to low titers in L cells without production of cytopathic effect CPE. After growing the virus in BHK-21 cells, however, the virus replicates to high titers in L cells with complete CPE. It was found that suspensions of MM virus propagated in L cells directly from the mouse brain contained much more interferon than did suspensions of virus which had first been grown in BHK-21 cells. Mouse brain suspensions of the virus were also found to contain high interferon titers. Treatment of L cells with actinomycin D before infection with mouse brain-grown virus resulted in full virus replication with CPE. BHK-21 cell-grown virus diluted in L cell interferon behaved like mouse brain-grown virus in L cells. It is concluded that the presence of interferon in the inoculum is largely responsible for the suppression of MM virus replication in L cells.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE