Accession Number:

AD0706558

Title:

THE INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES FOR THE TICK-BORNE ENCEPHALITIS VIRUS,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1970-05-11

Pagination or Media Count:

8.0

Abstract:

The indirect hemagglutination test IHA with native goose erythrocytes was used for the detection of antibodies to the tick-borne encephalitis virus. The relationship between the antibody titers in the IHA test and the conditions of sensitization and organization of the test were studied. Sensitization was effected via the incubation of a 2 suspension of erythrocytes in a phosphate buffer pH 5.8 - 6.0 with an equal volume of antigen for a period of 15 minutes at 37C, the sensitized erythrocytes should be added to the serum solutions in the form of a 0.33 suspension in a dextrose-gelatin-veronal buffer. The test may be conducted either at 4C or at room temperature. The IHA test is specific and is more sensitive than the conventional HI test. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE