COMPARATIVE PHYSIOLOGY OF PLEUROPNEUMONIA-LIKE AND L-TYPE ORGANISMS.
Rept. no. 1 (Final) 1 May 65-15 May 70,
SOUTH DAKOTA UNIV VERMILLION DEPT OF MICROBIOLOGY
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Several species of Mycoplasma and bacterial L-forms were examined with regard to comparative amino acid biosynthesis, membrane structure, nature and biosynthesis of lipids. Strains tested varied with respect to several amino acid synthetic pathways. Membranes of Mycoplasma laidlawii, strain B could be solubilized by a number of agents, among which anionic detergent gave rise to a seemingly homogeneous product on analytical ultracentrifugation, while succinylation yielded at least five fractions, apparently homogeneous by ultracentrifugation, but heterogeneous by analytical electrophoresis. Lipid was separable from protein. Mycoplasma sp., strain J, contains two glycolipids, cholesteryl beta-D-glucoside, synthesized from endogenous cholesterol and UDP-glucose, and 3,4,6-triacyl-beta-D-glucopyranose. Mycoplasma laidlawii, strain B, synthesizes monoglucosyl diglyceride and diglucosyl diglyceride from UDP-glucose and the appropriate receptor. On acidification of normal cultures, M. laidlawii synthesizes O-amino acid esters of phosphatidyl glycerol. D-adanine is the predominate amino acid. Author