INTERACTION BETWEEN MENINGOPNEUMONITIS ORGANISMS (MP) AND VESICULAR STOMATITIS VIRUS (VSV) WITH POLYMORPHONUCLEAR LEUCOCYTES (PMNL) AND MONOCYTES
Technical Report,01 Apr 1960,31 Dec 1969
SOUTHERN ILLINOIS UNIV AT CARBONDALE CARBONDALE
Pagination or Media Count:
A fluorocarbon extract of meningopneumonitis organisms FEM contained 7 subunits exhibiting precipitating, leucoagglutinating and CF activities. Immunological analysis of FEM showed 6 viral subunits. However, 11 components were observed by immunoelectrophoretic technique. Comparative immunological, biological and biophysical behavior of these antigenic preparations was described. A minimum of approximately 62 MP particles was required for leucoagglutination. Active leucoagglutination was demonstrated in FEM, and other extract of FEM and in MP wall preparations. Only 2 precipitinogens were involved in leucoagglutination. MP organisms were phagocyted and multiplied in rabbit pseudoeosinophils and mouse monocytes in vitro. Multiplication of MP was demonstrated by serial passages, by inhibition of growth by NA analogues and by microscopic examination of leucocytes stained with fluorescein conjugated MP-antibody. Phagocytosed and free organisms were transported to the liver, spleen and kidney. UV derived plaque type mutants of VSV were collected and studied comparatively for pathogenicity for mice and behavior in different cell lines. What appears to be host-induced modification occurred with 6 stable plaque mutants grown on HeLa. Electron microscopy of interaction of VSV and PMNL showed that virus is phaocyted in less than 15 minutes.