Accession Number:

AD0702744

Title:

RAPID LIQUID CHROMATOGRAPHY OF NUCLEOTIDES. THE SEPARATION OF PICOMOLE AMOUNTS OF 32Pi, AMP, ADP, AND ATP,

Descriptive Note:

Corporate Author:

NAVAL AIR DEVELOPMENT CENTER JOHNSVILLE PA AEROSPACE MEDICAL RESEARCH DEPT

Personal Author(s):

Report Date:

1969-12-30

Pagination or Media Count:

21.0

Abstract:

By means of high pressure pellicular anion exchange chromatography picomole amounts of 32Pi, AMP, ADP and ATP were separated in 25 minutes. The method uses a linear concentration gradient between 0.01 M-0.74 M in 30 minutes formed from a buffer containing 0.25 M KH2PO4 and 2.20 M KC2. The flow-rate was 24 ml per hour and the operating temperature was 70C. Author

Subject Categories:

  • Biochemistry
  • Physical Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE