BIOLOGICAL CHARACTERIZATION OF UNA VIRUS (COMMUNICATION 2)
FORT DETRICK FREDERICK MD
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Interest in the study of arboviruses has increased recently in connection with their isolation in many countries of the world. The present study was concerned with biological characteristics and development of optimal methods of introducing and preserving Una arbovirus belonging to Group A according to Casals classification. In the course of the study Una virus was found to produce CPE in VNK-21 tissue culture and plaques in chick embryo fibroblasts. The clinical pattern of the disease in white mice weighing 6-7 grams inoculated with Una virus intracerebrally is characterized by an acute development of the disease and paralysis of hind legs developing in 4-5 days after inoculation. The highest titers of the virus are found in the blood in 6 and 48 hours and in the brain in 72 hours after inoculation. Una virus may be preserved by lyophilization of the brain suspension with and without stabilizers in a vacuum-drying apparatus. Among stabilizers studied 5 peptone was found to be the best.