ANALYSIS OF METHANE AND CARBON MONOXIDE IN RESPIRATORY GASES BY GAS CHROMATOGRAPHY.
Medical research interim rept.,
NAVAL MEDICAL RESEARCH INST BETHESDA MD
Pagination or Media Count:
A method for the analysis of ambient inspired air and of respiratory gas samples for carbon monoxide and methane is described. The gas chromatographic procedure involves separation of methane and carbon monoxide from each other and from other gases by use of a 5A molecular sieve column. The effluent from the column is mixed with hydrogen, passed over a hot nickel catalyst, and then enters the flame ionization detector FID. Methane in the original sample is detected unchanged at the FID while carbon monoxide of the original sample is converted to methane in the catalyst bed and determined at the FID as methane. Volumes of respiratory gas samples from 2 to 10 ml. may be used depending upon the actual methane and carbon monoxide contents. Author
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