Accession Number:

AD0701251

Title:

GROWTH OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS IN HUMAN DIPLOID CELL STRAIN WI-38

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Reitman, Morton
• Green, Leonard

Report Date:

1969-10-08

Pagination or Media Count:

4.0

Abstract:

It was demonstrated that Venezuelan equine encephalomyelitis VEE virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 10 to the 9.8th power mouse intracerebral 50 lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects.

Descriptors:

• *TISSUE CULTURE CELLS
• *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS
• ARBOVIRUSES
• CULTURE MEDIA
• GROWTH(PHYSIOLOGY)
• VACCINES

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE