ENHANCED CO2 PRODUCTION BY YEAST CELLS EXPOSED TO ELEVATED TEMPERATURES.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
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The objective was to study the effects of increased temperatures upon cell functions and metabolic processes. After a period of starvation, cells exposed to elevated temperatures produced CO2 at a rate twice that of control cells. The lag period which precedes a linear rate of CO2 production by starved control cells was essentially eliminated in the heat treated cells and the uptake and retention of sorbose was greater in the heated cells. Exposures were accomplished by brief immersions in heated media and by growth for 2 hours at 35C instead of the usual 28.5C. Brief exposures were effective when glucose was included in the suspending medium exposures in water or growth medium without glucose resulted in a decreased production of CO2. The responses are similar to those obtained in other studies by incubating cells in sugar solutions. Author