Accession Number:

AD0699013

Title:

ENZYMIC DEGRADATION OF YEAST CELL-WALL MANNANS AND GALACTOMANNANS TO POLYMERIC FRAGMENTS CONTAINING alpha-(1-6)-LINKED D-MANNOPYRANOSE RESIDUES,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Report Date:

1969-05-06

Pagination or Media Count:

12.0

Abstract:

The cell-wall D-mannans of Candida parapsilosis, Endomycopsis fibuliger, Saccharomyces rouxii, Torulopsis apicola Hajsig strain, and Torulopsis bombi were degraded with an exo alpha-D-mannosidase from Arthrobacter GJM-1 to their alpha-1-6-linked D-mannopyranose main-chains, as demonstrated by p.m.r. spectroscopy. D-Galacto-D-mannans from Candida lipolytica, Torulopsis gropengiesseri, Torulopsis lactis-condensi, Torulopsis magnoliae, and Trichosporon fermentans could be degraded to polysaccharides containing mainly 6-O-linked alpha-D-mannopyranosyl residues following preferential removal of their enzyme-resistant, D-galactopyranosyl non-reducing end-units with acid. The D-mannans of Saccharomyces lodderi, Citeromyces matritensis, and Pichia pastoris could also be enzymically degraded to polysaccharides containing predominantly alpha-1-6-linked D-mannopyranosyl residues after hydrolysis of most of the beta-D-linked residues in their side chains with acid. The exo alpha-D-mannosidase, as would be expected, produced beta-D-mannose on splitting of an alpha-1-2-linked D-mannopyranose tetramer. Apparently a D-mannopyranose non-reducing end-unit and two consecutive alpha-D-mannopyranose residues are required by the enzyme for cleavage of a substrate to take place. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE