THE INTERACTION OF NUCLEIC ACIDS AND SPECIFIC ANTIGENS IN THE CELL DURING MIXED VIRAL INFECTIONS,
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Mixed viral infection, caused by the simultaneous administration of 2 viruses, was studied by the cytochemical method. Experimental infection was reproduced in white mice - this was done by the simultaneous administration of herpes virus and the virus of tick-borne encephalitis. With the application of acridine orange differences were observed in the staining of sections in the areas of Ammons horn and Purkinje cells of the cerebellum which were infected with the other virus or with both viruses simultaneously. In the case of herpes encephalitis these areas were primarily a green color, with tick-borne experimental encephalitis - yellow-orange, and with mixed infection - green-yellow. During mixed infection of white mice with the herpes and tick-borne encephalitis viruses a study was made of the localization and accumulation of antigens in the cells by the method of immunofluorescence. Specific luminescence during the different infections was characterized by the fact that with herpes encephalitis there was observed an earlier and more expressed fluorescence in the nuclei, nucle oli, and marginated masses, and with tick-borne encephalitis the cytoplasm fluoresced predominantly, luminescence of the nucle oli and uniformly distributed masses in the nucleus developed later and was less intensive. During staining of sections of animal brain following mixed infection a green fluorescence was observed in the nuclei, nucleoli, and in agglomerates which were marginated on the nuclear membrane, and also of large sectors of the cytoplasm which were adjacent to the nucleus, and a yellow fluorescence, inherent to rhodamine, was observed for the main mass of the cytoplasm.