INTENSITY OF PROTEIN SYNTHESIS IN THE MICROBIAL CELL AS A FACTOR DETERMINING THE EFFECT OF NEOMYCIN ON ITS METABOLISM
FORT DETRICK FREDERICK MD
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The inhibition of the effect of neomycins on the oxidation of glucose by non-multiplying bacterial cells is determined to a considerable degree by the conditions of their preliminary cultivation. Dependent on the latter is the synthesis of protein respiratory enzymes during the transfer of cells to a new medium and correspondingly the effect of neomycins, which are inhibitors of protein synthesis. Under conditions which are unfavorable for the incorporation of amino acids in protein in quiescent cells - in the absence of aeration and a source of energy in the medium, the difference between indices of incorporation of labeled methionine in the control and with the addition of neomycins is relatively small. Factors which intensify the synthesis of protein aeration and the presence of a source of energy in the medium, cause a much greater divergence between indices of incorporation in the test and in the control. Results of gasometric changes and tests with the incorporation of labeled precursors in protein show that the degree of disruption of biochemical processes in the microbial cell by neomycins depends mainly on the intensity of flow of protein synthesis in it.