CHANGES IN THE MUTAGENIC ACTION OF HYDROXYLAMINE ON T-2 PHAGE
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Treatment of extracellular T-2 bacteriophage with hydroxylamine at 60C led to an increase in the rate of induction of r- and hc-mutants in connection with an increase in the rate of the reaction of hydroxylamine with hydroxymethylcytosine in phage DNA due to a break in the hydrogen bonds and an increase in the initial rate of the reaction. The different increase in the rate of mutation in r- and h-genetic loci was conditioned by the heterogeneity of these areas based on G-HMC pairs of bases. The change in the course of the reaction of hydroxylamine with cytosine hydroxymethylcytosine in DNA of T-2 phage to the side of formation of uracil or thymine-like compounds, which to a greater degree than other intermediate compounds may be responsible for mutation changes, led to an increase in the rate of induction of r-mutations.