Accession Number:

AD0698862

Title:

CHANGES IN THE MUTAGENIC ACTION OF HYDROXYLAMINE ON T-2 PHAGE

Descriptive Note:

Journal article

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1969-10-30

Pagination or Media Count:

9.0

Abstract:

Treatment of extracellular T-2 bacteriophage with hydroxylamine at 60C led to an increase in the rate of induction of r- and hc-mutants in connection with an increase in the rate of the reaction of hydroxylamine with hydroxymethylcytosine in phage DNA due to a break in the hydrogen bonds and an increase in the initial rate of the reaction. The different increase in the rate of mutation in r- and h-genetic loci was conditioned by the heterogeneity of these areas based on G-HMC pairs of bases. The change in the course of the reaction of hydroxylamine with cytosine hydroxymethylcytosine in DNA of T-2 phage to the side of formation of uracil or thymine-like compounds, which to a greater degree than other intermediate compounds may be responsible for mutation changes, led to an increase in the rate of induction of r-mutations.

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE