Research Program for Alerting, Detection and Identification of Pathogens
Interim scientific rept. 1 Jul 68-1 Jul 1969
GENERAL ELECTRIC CO SYRACUSE NY ELECTRONICS LAB
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The objective of this program is to assess the feasibility of rapidly detecting and identifying pathogens when present in small populations. Gas chromatography is the methodology used. It was established that present methods of ether extraction and silylation of tissue culture supernatants and lysates are not conducive to obtaining reproducible results. Use of halogenated organic acids as media supplements offers promise of being a useful technique. Various methods of ether extraction of blood sera for indication of infection proved to be unsuccessful. Variation in incubation and extraction procedures was effective in altering the signature of bacteria. The effectiveness of the head space technique for obtaining microbial signatures was tested.
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