DEVELOPMENT OF AN ORALLY EFFECTIVE INSECT REPELLENT
Annual progress rept. 1 Nov 1968-31 Oct 1969
IIT RESEARCH INST CHICAGO IL
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Experimental observations were made to test the validity of the gamma-aminobutyric acid GABA-carbon dioxide CO2 hypothesis, designed to explain the neurochemical interactions that govern a mosquitos behavior in the presence of its host. It was postulated that the formation of carbamino-GABA underlies the activating effects of CO2 on mosquitoes. The isolated central nervous system of the cockroach, P. americana, was utilized to test the effects of carbamino-GABA and other compounds on the nervous system of an insect. GABA inhibited the spontaneous firing rate of this preparation in the absence of CO2, but stimulated it in its presence. Gamma-hydroxybutyric acid, a substance which cannot form a carbamino compound, was inhibitory both in the absence and presence of CO2. A chemical analogue of carbamino-GABA, N-acetyl GABA stimulated the preparation. It was shown that NAG and GABA probably compete for the same site on the synaptic membrane. The evaluation of selected compounds for mosquito repellency by the electronic recording method was studied. A statistically designed series of tests were performed to test the validity of some basic assumptions that are involved in using the electronic method for the assay of repellents.
- Hygiene and Sanitation