CHROMIUM COMPLEXES OF INSULIN AND RELATED COMPOUNDS.
Final rept. 1 Jun 63-31 Aug 69,
MARYLAND UNIV COLLEGE PARK DEPT OF CHEMISTRY
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The method of sequential dialysis has been developed by which biological compounds can be efficiently evaluated in this respect. The method has been used extensively in studying the reactions of CrIII, which is essential to the functioning of insulin, with many biological ligands including amino acids, bases, biological phosphates, most of the compounds of the Krebs cycle and many of the glycolytic chain. The most effective form compounds with CrIII having constant rates of transport through membranes for several days. Among these are citrate, isocitrate, mesoxalate, tartronate, ADP, ATP, thiamine pyrophosphate, histidine, fructose-1,6-diphosphate and 3-phosphoglycerate. Work in progress includes extension of these studies to additional biological ligands, different concentrations of reactants and essential metal ions other than CrIII. Author