BIOLOGICAL CHARACTERISTICS OF UNA VIRUS
ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA
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Interest in arbor viruses has heightened recently in view of the isolation of such viruses in many of the worlds countries. The present investigation deals with the biological distinctions and development of optimum methods for passing and preserving UNA arbor virus which is referable to group A according to the Casals classification. In the course of this study it was found that UNA virus induces CPE in VNK-21 tissue cultures and plaque production in chick fibroblast cultures. The clinical symptoms of pathology following infection of albino mice weighing 6-7 grams with UNA virus, intracerebrally, are characterized by acute development of disease, paralysis of the hind legs on the 4th-5th post-infection day. The virus is demonstrable in the blood in the highest titers 6 and 48 hours after infection, and in the brain 72 hours after infection. UNA virus can be preserved by means of lyophilization of a brain suspension with and without fillers in a vacuum dryer. Of those we studied 5 peptone was the best filler stabilizer.