EFFECT OF LASER MICRO-IRRADIATION ON ISOLATED CELLS.
Final technical rept. Apr 68-Jul 69,
COLLEGE DE FRANCE PARIS NATURALIA ET BIOLOGIA
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The work described was concerned principally with an examination of the effects produced by laser irradiation of isolated human blood cells and various types of cultured cells in an effort to study 1 the types of cellular damage produced by different wavelengths, energies, and types of laser emission 2 the possibility of destruction of specific cellular organelles, and 3 the reaction of other cells to an individual cells death. By comparing the thermal effects produced by laser light is stained cells with those induced in a stained model system albumin, it was found that it is possible to calculate the concentration of the stain in mitochondria. Evaluation of various parameters of laser irradiation particularly thermal events has led to the determination of the optimal irradiation times for both the measurement of local stain concentration and the selective destruction of infra-stained mitochondria. The ultraviolet laser has been used in preliminary experiments to demonstrate, at the ultrastructural level, that the morphological lesions produced in chromatin are very different from those observed after conventional UV irradiation, i.e., coagulation rather than paling is induced in the target area. Author