DIFFERENCES IN ANTIGENIC SPECIFICITY AND IMMUNOGENICITY OF TISSUE TRANSPLANT.
Rept. no. 1 (Final), Apr 68-Apr 69,
CANCER INST TOKYO (JAPAN)
Pagination or Media Count:
Antigenic specificity of four clonal sublines which were isolated by single cell transplantation from a rat ascites hepatoma, AH-64C, were compared by the technique of the Cross-Contact Transplantation CCT-Test, in which the peritoneal lymphoid cells previously sensitized with cells of each subline were transfered into syngeneic animals together with limited numbers of tumor cells. From survival curves of the hosts in the groups of different combinations, it was noted that the sensitized lymphoid cells showed an inhibitory effect on the cell proliferation of not only the subline used for sensitization but also other sublines, while lymphoid cells of normal rat or those sensitized with the normal rat liver showed no significant effect. The results suggested that the lymphoid cells, mostly lymphocytes, could recognize tumor-specific antigenicity different from the normal tissue, but could not strictly distinguish slight differences in cytological characteristics between the sublines, such as chromosome constitution or growth pattern, probably because of sharing of a common antigen among them. On the process of this experiment it was also pointed out that cells which survived in the resistant animals showed irreversible changes in their chromosome constitutions, suggesting that a possible selection or variation of cells having different antigenic specificity might be induced under immunologically modified conditions of the host animals. Author
- Anatomy and Physiology
- Medicine and Medical Research