DIFFUSIONAL PHENOMENA IN FLOWING POLYMER SOLUTIONS. I. THEORY OF RESOLUTION DISPERSION IN GEL PERMEATION CHROMATOGRAPHY.
STEVENS INST OF TECH HOBOKEN N J DEPT OF CHEMISTRY AND CHEMICAL ENGINEERING
Pagination or Media Count:
A systems approach is applied to the analysis of chromatogram resolution dispersion, or zone broadening, in Gel Permeation Chromatography G. P. C.. Three possible sources of dispersion are considered these are the packed columns, the empty tubing between pump and columns, columns and detector, etc., and the detection system, vis., the differential refractometer cell. It is shown that empty tubing can contribute significantly to the degree of dispersion and to skewness of elution curves and that this dispersion should depend on molecular weight of solute polymer and diameter and length of the tubing. The importance of dispersion in the empty tubing is compared with that in the packed columns and refractometer cell. Author
- Physical Chemistry