ANTIBIOTIC AND CHEMOTHERAPEUTIC AGENT TRANSPORT MECHANISMS IN BACTERIAL MEMBRANES.
Rept. no. 4 (Annual summary), 1 May 68-30 Jun 69,
PHILCO-FORD CORP NEWPORT BEACH CALIF AERONUTRONIC DIV
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Procedures were developed for the analysis of permeability-related mechanisms of resistance to antibiotics and chemotherapeutic drugs in bacteria, utilizing model membranes and modified bacterial preparations. Lipid bilayer membranes and liquid crystal spherules, or liposomes have been used to analyze diffusional transport relations. Bacterial spheroplasts, obtained by enzymatic stripping of the cell walls, were used in the examination of active transport relations. Basic transport relations were examined using the model drugs, indole-3-ethanol, indole-3-acetic acid and tryptamine, as neutral, acidic and basic transport solutes. Chlortetracycline CTC was the model antibiotic substance. The lipid bilayer membrane studies suggest that the nature and charge of the amphipathic lipid forming a membrane may have a major role in governing diffusional permeability in cells but are probably not significantly involved in the resistance mechanisms of the test organisms. Macromolecular components of the cell appear to have a significant effect on CTC permeability. Author