AFLATOXINS IN MAMMALIAN CELL CULTURES
TUSKEGEE INST AL CARVER RESEARCH FOUNDATION
Pagination or Media Count:
KB and HeLa mammalian tissue culture cells were cultured in the presence of 0.4, 1, and 4 ppm of aflatoxin. The incorporation of labeled uridine into the different RNA components separated by sucrose gradient ultracentrifugation and by methylated albumin column chromatography was studied. All levels of aflatoxin increased the proportion of incorporation into RNA in the 4s region after 4 hours and 12 hours of exposure. The longer the time of exposure the more severe was the effect. It is not known whether this is the result of depolymerization or of stimulation of t-RNA synthesis or both.