EFFECTS OF CYANIDE AND 2 DEOXYGLUCOSE ON PROXIMAL TUBULAR FUNCTION IN THE RAT KIDNEY
ARMY AEROMEDICAL RESEARCH LAB FORT RUCKER AL
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A series of 13 experiments were performed to study the effects of cyanide, an oxidative inhibitor, and 2 deoxyglucose 2 DG, a glycolytic inhibitor, on the function of the proximal tubule of the rats kidney. The technique utilized was split oil droplet microperfusion of surface proximal nephron segments with sequential photomicrography. Isotonic saline was the control perfusion fluid. Cyanide reduced the reabsorptive rate of the perfused nephron segments to 50 of the control. 2DG had no effect on saline reabsorption. Cyanide plus 2DG perfused simultaneously in saline inhibited reabsorption to the same degree as did cyanide alone. These results are interpreted as indicating almost total dependence of proximal tubular reabsorption of filtrate upon energy available from oxidative metabolism. Since reabsorption of filtrate in this segment is medicated through active sodium transport, it would appear that oxidative metabolism and not glycolysis is the energy source for this process.
- Anatomy and Physiology