MORPHOLOGICAL CORRELATIONS INVOLVED IN THE DIFFERENTIATION OF THE EYE LENS.
Progress rept. no. 4 (Final) 1 Jul 68-30 Jun 69,
GRANADA UNIV (SPAIN) DEPARTAMENTO DE ANATOMIA
Pagination or Media Count:
On the basis of immunological techniques, cellulose acetate and starch gel electrophoresis, differences in macromolecular composition of the lens cells were detected in correlation with the age and cytological differentiation. From the three major groups of soluble lens proteins alpha, beta, and delta crystallins the last fraction should apparently be considered as a transient antigen. In fact, delta crystallin progressively disappears from the equatorial and cortical cells in relation to aging. Furthermore DC was detected at a higher level in the embryonic core of the organ, and in the epithelial cells after experimental lens rotations. Other dynamic changes of soluble crystallins, suggest that a differential macromolecular biosynthesis exhists during aging and cytological differentiation of the lens. Biochemical and immunological analysis of the lens in the chick, during development, provides qualitative data of genetic expression during the ontogenic differentiation of the organ. This information could be compared with those obtained under experimental conditions in order to understand the mechanism involved in gene regulation of the lens. Author
- Anatomy and Physiology