Accession Number:

AD0689097

Title:

PURIFICATION OF RAT GAMMA GLOBULIN AND THE PRODUCTION OF A SPECIFIC ANTI-RAT GAMMA GLOBULIN SERUM

Descriptive Note:

Technical note

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Personal Author(s):

Report Date:

1969-03-01

Pagination or Media Count:

24.0

Abstract:

A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 has been presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of the fraction when tested against anti-rat sera confirms the homogeneity of this 7S fraction. A monospecific anti-serum to rat 7S gamma globulin has been produced. This antiserum is free of reaction with all other serum components when subjected to immunoelectrophoresis against normal rat serum.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE