EFFECT OF X RAYS AND 60Co GAMMA RAYS ON THE LIVER ENZYME SYSTEM RESPONSIBLE FOR FATTY ACID SYNTHESIS
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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The report describes effects of in vivo exposure to x rays and 60Co gamma rays on the fatty acid synthesizing liver enzyme system. Both fed and fasted young female Sprague-Dawley rats were utilized in these studies. All irradiated animals received a single whole-body exposure of 1200 R at 20 Rmin. The irradiated animals as well as sham irradiated controls were sacrificed at predetermined times after exposure and cell-free liver homogenates which contained the enzyme system under investigation were prepared. It was found that the activity of the liver enzyme system responsible for the biosynthesis of fatty acids is greatly stimulated by x or 60Co gamma rays and that the cytoplasmic soluble enzymes are affected by radiation to a much greater extent than the mitochondrial enzymes. Blood glucose determinations were carried out in irradiated and control rats as well as in rats which were made alloxan diabetic. The results indicate that the enhancement of enzyme activity observed is not due to an increase in the blood glucose level of the animal as a result of exposure to radiation but to some other factor the nature of which has not yet been elucidated.