KREBS CYCLE DEHYDROGENASE SYSTEMS ACTIVITY IN THE GASTROINTESTINAL TRACT OF RATS AFTER WHOLE-BODY IONIZING IRRADIATION
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Using a tetrazolium salt, three of the Krebs cycle dehydrogenase systems were studied in homogenates of four regions of the gastrointestinal tract of the rat following either x rays or mixed gamma-neutron radiation delivered to the whole body WBR. Microchemical assays were done at intervals after irradiation 10-20 minutes, 1, 2, 3 days. There was a fall in activity as early as 10-20 minutes postirradiation which increased in magnitude by the second and third day, for all regions studied. The gamma-neutron irradiations were relatively more effective than were the x rays in bringing about this depression in malate-, succinate- and isocitrate- dependent dehydrogenase activity. These studies clearly indicate that the activity of the three assayed Krebs cycle dehydrogenase systems in the regions of the gastrointestinal tract studied was below normal after exposure to WBR. The functional capacity of the Krebs cycle appears impaired which may account, in part, for some of the functional derangements seen in such animals.