MEMBRANE CHANGES IN YEAST CELLS CAUSED BY SULFHYDRYL REAGENTS AND ACCOMPANIED BY A SELECTIVE RELEASE OF SUGAR.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
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An analysis was made of the effects of heat on cells by studying the mechanism involved in sugar retention by yeast, a phenomenon which is increased, along with an enhancement of CO2 production, by exposure of cells to increases in temperature. Iodoacetic acid or N-ethylmaleimide included in cell suspensions induce a complete efflux of sorbose in contrast to a usual two-phase exit and some retention. Pretreatment produces cells capable of uptake and complete exit. Microscopically, cell vacuoles are disrupted before methylene blue penetrates and stains the cells. Vacuoleless cells also allow increased sorbose efflux. Author
- Anatomy and Physiology