Accession Number:

AD0688372

Title:

SINGLE VS. DOUBLE ANTIBODY RADIOIMMUNOASSAYS FOR PLASMA INSULIN,

Descriptive Note:

Corporate Author:

SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Report Date:

1969-03-01

Pagination or Media Count:

11.0

Abstract:

The double antibody assay and the charcoal assay for plasma insulin were compared as to sensitivity, expense, convenience of use, and adaptability to assay other hormones. The double antibody assay had an overall sensitivity of 0.360.1 micro units, with a sensitivity in the 0 to 1 micro units range of 1.20.1 micro units. The charcoal assay sensitivity was 0.250.1 micro units. The double antibody assay detected down to 0.8 micro unitsml in contrast to 4 micro unitsml. with the charcoal assay. The double assay cost twice as much as the charcoal assay, took more man-hours to complete, and was more difficult to use. The charcoal assay was superior for long-term, high-volume use, and was more easily adaptable to the assay of other hormones. Author

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE