A SIMPLIFIED METHOD OF TRYPSINATION AND GROWING OF RABBIT KIDNEY CELL MONOLAYER CULTURES
ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA
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The authors recommend the hot 37 degrees single-step method for trypsinization of kidneys of baby and adult rabbits. This method was found to be superior to others tried in yield of cells per gram of tissue, trypsin consumption, growth achieved, and economy. The use of Hottinger muscle digest as the nutritive medium for cultivation insures great availability of rabbit cultures for institutes and practical laboratories. The use of monolayer rabbit kidney cultures in veterinary virology is promising because of the possibility of creating standard conditions for obtaining tissue.