STUDIES ON THE EMBRYOLOGY, PIGMENTS AND GLUCOSE METABOLISM OF THE marine borer, BANKIA SETACEA.
Final rept. 1 Apr 64-31 Mar 69,
BRITISH COLUMBIA RESEARCH COUNCIL VANCOUVER
Pagination or Media Count:
B. setacea was reared in the laboratory, from the germ cell stage to larval metamorphosis. Asepsis, by tissue culture techniques and antibiotics, was essential. Sexual differences in exhalant siphons of B. setacea were observed, as well as instances of internal fertilization. Fertilized ova are more dense than sea water. Certain external conditions, particularly salinites below 12 ooo, caused loss of control of organized development in fertilized ova. Large masses of single cells, even motile ciliated cells, were produced. The pigment contained in B. setacea siphons was demonstrated to be an iron porphyrin, indistinguishable, on the basis of spectra and chromatographic behaviour, from sheep blood protoporphyrin IX. The presence of a number of intermediate metabolic pathways in the marine-borer caecum was demonstrated, including the Embden-Meyerhof pathway, the pentose cycle, the citric acid cycle and the non-triose phosphate pathway. Intermediate compounds were identified and specific activity of enzymes was measured. The pentose cycle accounted for 3 of glycolysis in the caecum. A short one-step assay for cellobiase was developed. Author
- Anatomy and Physiology
- Biological Oceanography