Accession Number:

AD0686274

Title:

THE USE OF AZO DYE-BOUND COLLAGEN TO MEASURE THE REACTION VELOCITIES OF PROTEOLYTIC ENZYMES.

Descriptive Note:

Interim rept.,

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

Report Date:

1969-02-20

Pagination or Media Count:

12.0

Abstract:

The insoluble protein-dye complex, Azocoll, can be used to measure proteolytic activity as a function of time. By using the aliquoted method discussed in this report, one 5.0 ml reaction mixture can give up to five points on a standard velocity curve. It has been shown that the ratio of solid to liquid in the reaction mixture can be kept constant to give accurate values when pipetting aliquots from a solid-liquid mixture. We have also shown that Azocoll is more sensitive to trypsin than previously reported and that the dye bleeding in control samples can be kept to a manageable level. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE