Accession Number:

AD0686140

Title:

ADVANTAGES OF PROPAGATING RABIES VIRUS IN ROLLER SUSPENSION CULTURES IN CELL LINE BHK-21 WHEN USING THE METHODS OF SUBCULTURES AND MIXED CULTURES OF INFECTED AND NON-INFECTED CELLS,

Descriptive Note:

Corporate Author:

ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s):

Report Date:

1969-03-06

Pagination or Media Count:

10.0

Abstract:

A method is proposed for propagating rabies virus in roller-suspension cultures, instead of the standard stationary monolayer techniques. Both the subculture and cell-mixing methods were used with hamster kidney fibroblasts of the VNK-21 line. Primary infection of cells was achieved by addition of a 10 suspension of infected brain, and after nine passages, virus titers up to 10 to the 7.29 power LD500.03 ml were achieved. The advantages of the roller-suspension method are 1 higher virus titers 2 accumulation of maximum titers 40 faster than in monolayers 3 easier detection of specific rabies virus antigen by immunofluorescence, appearance of cytoplasmic incorporations and development of a cytopathic effect. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE